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Fragment ion spectra were generated using enhanced data-independent ion mobility (HDMSE) acquisition mode where data from mobility separation ions are collected in two channels with either low collision energy applied at 4 V or with an elevated collision energy ramp from 25 to 60 V for precursor and fragment ions, respectively

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Of the homozygous mutations most studied, a carrier of the homozygous T677T isoform produces 75% less methyl folate than someone without the mutation, and carriers of the homozygous C1298C isoform produces 17% less methyl folate (Table 1)