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For soft agar colony-forming assay, HeLa cells (1.5 × 10 3 ) were plated in 0.6% agarose solution and layered on 1.0% agarose bed before treatment with different concentrations of metformin, DCA or both in combination

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(2014, PMID 23755725) reported effects on the nitric oxide system in rodent models, and Chang et al

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