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Hives can be triggered by physical factors such as heat, cold, exercise, sunlight, stress, sustained pressure on a skin area (such as from a belt or shoulder strap), a sudden increase in body temperature (from a fever or a hot bath or shower) or from an irritating chemical, cosmetic or soap applied to the skin

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After several washes with distilled water, bands corresponding to PDHc-E2 (~75 kDa) or OGDHc-E2 (~55 kDa) were excised using a sterile scalpel and transferred to Protein LoBind tubes (Eppendorf)