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Population Doubling Level Calculation To calculate the PDL, a formula was used as follows: n = 3.32 (log UCY −log I ) + X , where n is the final PDL at the end of a given subculture, UCY is the cell yield at that point, I is the cell number used as inoculum to begin that subculture, and X is the PDL of the inoculum used to initiate the subculture being quantitated ( SA-β-Galactosidase Staining Cells were seeded in 6-well microplates at 4 × 10 4 cells/well