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For lentiviral expression of GESP, either the pLVSIN-CMV Pur vector (Takara Bio Inc.) encoding HA-tagged GESP or an empty vector control was transfected into Lenti-X293T cells (Takara Bio Inc.) together with Lentiviral High Titer Packaging Mix (Takara Bio Inc.)

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1,2 Each of these definitions carries a different meaning, and what the half-life of a drug is found to be can also vary or appear to vary depending on contexts such as formulation, route of administration, and individual variations in drug distribution, metabolism, and excretion, among other factors