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For promoter analysis, 2 kb upstream region from translation start code ATG of each GhGPXs was from database of CGP 39 and another database 41 , and then predicted cis -acting elements in (+) strand and (−) strand of each promoter of GhGPXs was found using the database of PlantCARE 42 (Plant Cis -Acting Regualtory Element, Gene expression analysis Total RNA was extracted by using TRIzol reagent (Invitrogen) from G

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Compounded MIC with L-Carnitine and B1/B2/B6 in a 10ml vial (Methionine 12.4, Inositol 25, Choline 25, Carnitine 125, B1 50, B2 5, B6 2 mg/mL)