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The 24-h biofilms (prepared as described above) were washed twice with 200 μl of PBS and then further incubated for 24 h at 37°C in an atmosphere of 5% CO 2 and 95% humidity, with 100 μl of RPMI 1640 medium (control) or with ΔM3 or ΔM4 at a concentration ranging from 1.6 μM to 100 μM in the same medium

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